Following is a list of transfers of the 2009 Paraguayan Primera División season. The División Profesional de la Asociación Paraguaya de Fútbol (), also known as the Primera División (), or due to sponsorship reasons Copa TIGO, is the top-flight professional football league in Paraguay. There are 12 teams in the first division.
Cerro Porteño In: Jorge Daniel Núñez from Club Nacional (Paraguay) Ivan Gonzalez Ferreira from Sol de America Julio Dos Santos from Atletico Paranaense Jaison Ibarrola from Universidad Católica Carlos Recalde from Argentinos Juniors Roberto Nanni from Vélez Sársfield
Out: Germán Castillo from Gimnasia y Esgrima de Jujuy Alcides Piccoli to Gimnasia y Esgrima de Jujuy Roberto Junior Fernandez to Estudiantes de la Plata Jonni Cabrera to Udinese Walter Fretes to Sportivo Luqueño Carlos Villagra to Sportivo Luqueño Alfredo Rojas to Club Nacional (Paraguay)
Olimpia In: Claudio Vargas & Daniel Ferreira from Sportivo Luqueño Carlos Bonet from Cruz Azul Nelson Romero from Club Libertad Pablo Giménez from Querétaro FC Nelson Cuevas from Universidad de Chile
Out: Denis Caniza out to Club Nacional