A mayfly is a type of insect belonging to the order Ephemeroptera.
Mayfly or Mayflies may also refer to:
Music Mayfly, an album by Cy Curnin "Mayfly", a song by Belle and Sebastian from If You're Feeling Sinister Mayfly (band), an American Christian metal band from Ohio The Mayflies USA, an American power pop band
Film, television, literature Mayfly (film), a 2008 short film Mayflies (TV series), a 2022 two-part television drama Mayflies (novel), a novel by Kevin O'Donnell Jr. Mayflies, a novel by Andrew O'Hagan
Transportation HMA No. 1 or Mayfly, a British Royal Navy airship HMS Mayfly, several British Royal Navy ships Bland Mayfly, an early aircraft constructed in 1910 by Lilian E. Bland Halton Mayfly, a British two-seat biplane designed by C.H. Latimer-Needham in the 1920s Southend MPG Mayfly, a British two-seat human-powered aircraft, built in the 1960s