The Dutch Eerste Divisie in the 1964–65 season was contested by 16 teams. Willem II won the championship for the second time.
New entrants Promoted from the 1963–64 Tweede Divisie: Alkmaar '54 NEC Relegated from the 1963–64 Eredivisie: Blauw-Wit Amsterdam FC Volendam
League standings
Championship match Willem II & Elinkwijk played a match to determine the league champions (on the field of BVV).
See also 1964–65 Eredivisie 1964–65 Tweede Divisie
References Netherlands - List of final tables (RSSSF)