In public transport, Route 3 may refer to:

Route 3 (MTA Maryland), a bus route in Baltimore, Maryland and its suburbs Barcelona Metro line 3 London Buses route 3 Line 3 (Madrid Metro) Melbourne tram route 3 3 (New York City Subway service) Seoul Subway Line 3 3 Jackson, a bus route in San Francisco Shanghai Metro Line 3 Southern Vectis route 3, a bus route running between Newport and Ryde on the Isle of Wight

See also Line 3 (disambiguation)