Tsamis () is a Greek surname. Notable people with that surname include:
Angelos Tsamis (born 1981), Greek professional basketball player Dionysis Tsamis (1951–2026), Greek football player George Tsamis (born 1967), American baseball player, coach, and team manager Lazaros Tsamis (1878–1933), Aromanian revolutionary Mikes Tsamis (1895–unknown), Greek water polo player