Holstein is a German and Danish surname, often used with the nobiliary particle "von", meaning "of", and may refer to:
Anna Morris Holstein (1824–1901), American organizational founder, civil war nurse, author Barry Holstein (born 1943), American physicist Betty Holstein, Australian tennis player Casper Holstein, (1877–1944) American mobster Denise Holstein (1927–2024), French Holocaust survivor and witness Eli Holstein (born 2004), American football player Franz von Holstein (1821–1878), German composer Friedrich von Holstein (1837–1909), German statesman Helvig of Holstein (1260–1324), a Swedish queen Isaac Holstein (born 1987), Filipino-American basketball player John C. Holstein (1945–2024), American judge of the Supreme Court of Missouri Mechtild of Holstein (1220 or 1225–1288), a Danish queen consort Theodore Holstein (1915–1985), American theoretical physicist
See also Staël von Holstein Schleswig-Holstein