Operation Polka Dot was a U.S. Army test of a biological cluster bomb during the early 1950s.

Operation Operation Polka Dot was a field test of the E133 cluster bomb undertaken at Dugway Proving Ground in Utah during the early 1950s. The operation was detailed in a July 18, 1955 U.S. Army report that also detailed Operation Trouble Maker.U.S. National Research Council, Subcommittee on Zinc Cadmium Sulfide. Toxicologic Assessment of the Army's Zinc Cadmium Sulfide Dispersion, (Google Books), National Academies Press, 1997, pp. 44-52, (). The operation was classified "secret"Committee on Foreign Affairs, Subcommittee on National Security Policy and Scientific Developments. "U.S. Chemical Warfare Policy", (Google Books), 93rd U.S. Congress - 2nd Session, U.S. Government Printing Office, 1974, p. 340. and involved filling the munitions with the biological agent simulant, Bacillus globigii.

See also Operation Dew Operation LAC

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