Route 540, or Highway 540, may refer to:
Canada Alberta Highway 540 New Brunswick Route 540 Ontario Highway 540 Ontario Highway 540A Ontario Highway 540B Quebec Autoroute 540
United Kingdom A540 road
United States Interstate 540 (disambiguation) Arkansas Highway 540 (former) Florida State Road 540 County Road 540 (Polk County, Florida) Georgia State Route 540 Hawaii Route 540 Maryland Route 540 County Route 540 (New Jersey) North Carolina Highway 540 Ohio State Route 540 Oregon Route 540 Virginia State Route 540 (1931) Puerto Rico Highway 540 Washington State Route 540