WLS may refer to:
Arts, entertainment, and media WLS (AM), a radio station in Chicago, Illinois, US WLS-FM, a radio station in Chicago, Illinois, US WLS-TV, a television station in Chicago, Illinois, US DWLS, a radio station in Metro Manila, Philippines
Biology and healthcare GPR177, Wntless, or WLS, a human gene Weight loss surgery
Computing and technology Oracle WebLogic Server, a Java application server White light scanner, for measuring surface height Wavelength shifter, material that absorbs a wavelength and emits another
Other uses Wisconsin Lutheran Seminary, in the United States WLS, the Chapman code for Wales Weighted least squares, in statistics West London Synagogue Westchester Library System, New York, United States Women's League Soccer (2011–13), a soccer league in the United States