Lotis can refer to: Lotis (mythology), a nymph in Greek mythology Lotis (beetle), a genus of beetles Large Optical Test and Integration Site, at the Lockheed Martin Space Systems Company in Sunnyvale, CA Livermore Optical Transient Imaging System, an automated telescope for follow-up of gamma ray bursts LOTIS committee, the Liberalisation of Trade in Services Committee, a working group within TheCityUK, United Kingdom 429 Lotis, a main belt asteroid
People with the name Lotis Key, former Filipino-American professional film and theater actress Dennis Lotis (1925–2023), South African-born British singer, actor, and entertainer