The antagonists of the Reborn! anime and manga series, known in Japan as Katekyō Hitman Reborn!, are created by Akira Amano. Set in a fictional town called Namimori in modern Japan, the series' main protagonists are of Japanese descent. However, their connection to an Italian Mafia family results in a majority of other characters introduced being mainly of Italian descent, including most of its antagonists.

The story centers on middle school student Tsunayoshi "Tsuna" Sawada, who finds out that he is the heir to the Vongola crime family. Reborn, the titular character and infant hitman, is sent to train him, and slowly recruits members into Tsuna's own Mafia family. They eventually attract attention from those who want to annihilate the future boss of the Vongola Family. Enemies such as the Kokuyo Gang, a group of Mafia criminals, and The Varia, an elite team of assassins, prove to be formidable challenges for Tsuna and his comrades. When some of the characters are later transported into an alternate future, nine years and ten months later, they have to adapt to a world where every Vongola member, as well as their allies, is being hunted down by a powerful rival family, the Millefiore. After returning from the future, they have to fight against an allied Family of Vongola since first generation who's seeking revenge to Vongola, Simon Family who turned out to be manipulated by Vongola Primo's Mist Guardian, Demon Spade who intended to rebuild the Vongola that he desire. Later, Tsuna and the others participate in Representative Battle of the Rainbow under request from the Arcobaleno with the winner will be able to have his or her curse removed. However, things started to get worse once an Arcobaleno with a clear pacifier, Bermuda von Veckenschtein appears with his team and participate the Representative Battle in order to get his revenge on Checker Face at the cost of the Arcobaleno's lives.

Kokuyo Gang

The Kokuyo Gang is a group led by Mukuro Rokudo, who formed the group after escaping from a special, high security jail in Italy which housed heinous criminals who have even committed crimes against the Mafia. Along with the two comrades he escaped with, Ken Joshima and Chikusa Kakimoto, he recruited other Mafia criminals who were able to break out from the same jail some time later. After their defeat at the hands of Tsuna and his friends, they were all once again imprisoned, though Chikusa and Ken are later able to escape, while Mukuro strikes a deal with the Vongola for their safety.

Mukuro Rokudo

is a 15-year-old Mafia criminal who escaped from an Italian prison, which held some of the most dangerous Mafia criminals, before he was to be executed. Not long after, he, along with his two subordinates, came to Japan, enrolled in Kokuyo Junior High, and started the Kokuyo Gang, which consists of heinous criminals who have recently escaped from prison. Skilled in casting illusions as well as mind control, he attempts to take his revenge on the Mafia by taking over Tsuna's body and using him to wreak havoc. When his plan fails due to Tsuna's interference, he is once again imprisoned. However, later on in the series, after possessing the body of Chrome Dokuro, he takes on a more supportive role for the series' main characters, though still prefers to not associate with them. He is voiced by Toshinobu Iida in the anime.

Ken Joshima is a 14-year-old loyal friend and follower of Mukuro. Born on July 28, he was also experimented on by the Estraneo Family, and decided to follow Mukuro when the boy killed their captors. The experimentation on him resulted in his ability to gain the attributes of certain animals, like wolves and lions, when he changes to different sets of teeth, increasing his agility, strength, and senses. He was Chikusa's cellmate in jail and often calls him "Kakipi". He is defeated by Takeshi Yamamoto and later defeated once again by an injured Kyoya Hibari. He is taken away by the Vendicare, but later escapes. Ken returns for the Vongola Tournament, serving as a bodyguard for Chrome Dokuro, whom he is fond of despite he often mocks and berates. He is voiced by Ryō Naitō in the anime.

Chikusa Kakimoto is a 14-year-old loyal friend and follower of Mukuro. Born on October 26, he, along with Mukuro and Ken, was experimented on by the Estraneo Family, and accepted Mukuro's offer to come with him when the latter killed their captors. He uses a pair of metal yo-yos that shoot poisonous needles as his primary weapons. After arriving in Namimori, he loses a battle against Hayato Gokudera, though finds out the identity of the tenth Vongola boss. Later, after sustaining further injuries by Kyoya Hibari, he is taken away and imprisoned by the Mafia's guardians, the Vendicare, along with the rest of the gang. He and Ken are able to successfully escape, with Mukuro ensuring their safety by striking a deal with Iemitsu Sawada. He returns for the Vongola Tournament, serving as a bodyguard for Chrome Dokuro, whose well-being is of concern to both him and Ken despite his open dislike for her. He is voiced by Toshiyuki Toyonaga in the anime.

Chrome Dokuro Chrome Dokuro is a loyal 13-year-old vessel of Mukuro and one of the newest members of the gang. She is originally a girl named Nagi who was found by Mukuro when on the verge of death after her attempt to rescue a cat from traffic accident at the cost of her right eye and several of her organs. Mukuro allowed her to live by creating illusionary organs for her with his illusion, intertwining their existence. She became co-Mist Guardian of Vongola, fighting against Mammon in the Ring Conflict, which result on her lost until Mukuro switched place with her and defeats Mammon. A week after Mukuro's release from Vendicare prison, she was kicked out from the gang by Mukuro and begins living on her own in Namimori with Tsuna and his friends. The reason of her discard was revealed during the third day of Representative Battle; it was because of her own dilemma between wanting to be acknowledged as individual and wanting to keep living with Mukuro's illusionary organs, causing her health deteriorating. This left Mukuro no choice but to keep her at distance from him. However, she's able to overcome her dilemma and recreate her organs with her own power, defeating two Vindices with hers and Mukuro's combined power. As she and Mukuro shared one body, she's able to use two of Mukuro's six skills. However, once Mukuro was freed from Vendicare Prison, she can no longer use this ability. She used to use the same trident like Mukuro as her main weapon, but after Mukuro was released, the spikes of her trident reduced to one. She is voiced by Satomi Akesaka in the Anime.

M.M. is a 15-year-old Mafia criminal who broke out of prison after Mukuro escaped. Born on July 3. She believes that money is the most important thing. After her defeat at the hands of Bianchi, she is later taken away by the Vendicare, but later rejoined the gang after Mukuro is released from prison. Her weapon is a clarinet that is constructed like a microwave oven. When played, the clarinet creates special sound waves that cause atoms to vibrate at an incredible rate which results in the substance the clarinet is aimed at to boil instantly. She calls the attack Burning Vibrato. When in close range combat, her clarinet splits, becoming a pair of nunchaku. She is voiced by Shōko Ishii in the anime.

Lancia is a 25-year-old Mafia criminal initially mistaken as Mukuro Rokudo. Born on December 15, as a child, he was adopted by the leader of a Mafia family in Northern Italy. The same family would later take in a young Mukuro, who periodically mind controls Lancia, and even makes him slay his entire family. Lancia eventually falls under Mukuro's complete control, losing his will to fight and forgetting his own name, and is soon captured and jailed. After Tsuna defeats him and Mukuro, he is freed from the latter's control, and is taken away by the Vendicare. He returns to Japan during the Vongola Tournament, defeating most members of the Varia's lower members, and being revealed as the strongest person in Northern Italy. Apparently, he became sensitive to Mukuro's thoughts due to being possessed by him so many times. He leaves for Italy after giving Tsuna his own family's ring. Lancia uses a giant steel ball and chain as his primary weapon, which he launches at the enemy using palm thrusts. Due to the ball's snake-like engravings on the surface, it can track and redirect air currents that create a vortex, sucking in whatever is near, and allowing Lancia to change its rotation in mid-air. He is voiced by Takeshi Maeda in the anime.

Birds Birds is one of dangerous criminals that assisted Mukuro on finding Tsuna. He is accompanied by yellow birds that always followed him around and double as a signal to Mukuro if he was defeated. He is an old man who finds pleasure in suffering of others, as he has nosebleeds when he ordering Tsuna's friends to beat him up. He ordered the Bloody Twins to follow Kyoko and Haru and threatened Tsuna and his friends that they will kill them if they didn't listen to what he said. However, his plan was foiled due to Shamal, Future Lambo and I-Pin's interference, resulting with his defeat and he was later arrested. He was voiced by Takahiro Hirano in the anime.

Bloody Twins Gigi and Didi are a pair of brutal serial killers that loyal to Birds. They are ones of criminals that Mukuro had freed along with him, becoming part of Kokuyo Gang members. They are used as a blackmail, following Kyoko and Haru and threatened to kill them if Tsuna and his friends don't listen to Birds' order. However, they were defeated by Shamal and Future I-Pin who have been asked by Reborn to act as the two girls' bodyguards. They were arrested along with the other members afterwards.

The Varia

The Varia is the Vongola Family's elite, independent assassination team. They are composed of genius assassins who work in the deepest recesses of the Mafia. Each member takes on missions that are said to be impossible to accomplish by humans, however, they do not accept a mission unless they believe there is a 90% chance of success. Those that see them work often say that their high level assassination skills are demonic. Despite lacking loyalty and respect for other members, they are extremely loyal to their leader, Xanxus. The Varia also appear to have more than fifty subordinates ranged under the command of the main members. The Varia is apparently reclusive, only showing themselves after Iemitsu Sawada disappeared.

During the alternate future arc, a 32-member Varia squad led by Xanxus attack a Millefiore base in Italy in accordance with the Vongola's plan to eliminate the Millefiore. Despite their fierce loyalty to the Vongola Family, the Varia only consider themselves as the Vongola IX's assassination team, still not acknowledging Tsuna to be the rightful Vongola X.

Xanxus