Tsetse flies are one of the large biting flies inhabiting much of mid-continental Africa between the Sahara and the Kalahari deserts.
Tsetse may also refer to: Tse Tse Fly (band), a late 1980s/early 1990s British rock band De Havilland Mosquito, FB Mk XVIII version was nicknamed Tsetse Tsetse (nuclear primary), the common design nuclear fission bomb core for several Cold War designs for American nuclear and thermonuclear weapons Tsetse, South Africa, a village in South Africa