Mishmar Ayalon () is a moshav in central Israel. Located between Latrun and Ramla on the old Jerusalem-Tel Aviv road, it falls under the jurisdiction of Gezer Regional Council. In it had a population of .
Etymology The moshav overlooks the Biblical valley of Ayalon (Joshua 10:12), after which it is named.Carta's Official Guide to Israel and Complete Gazetteer to all Sites in the Holy Land. (3rd edition 1993) Jerusalem, Carta, p. 333,
History The moshav was founded in 1949 by a gar'in of Jewish Holocaust survivors from Czechoslovakia, on land located near the depopulated Palestinian Arab village of Al-Qubab.
The Lehi forest is located in the moshav. In 2005 a monument to Lehi fighters (the Stern Gang) was erected in the village.Lechi monument in the village of Mishmar Ayalon Israel and You
The moshav's main industry is agriculture, particularly focusing on fruit, vegetables and dairy farming known for their high quality cheese.
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