Holofernes was an Assyrian invading general of Nebuchadnezzar, who appears in the deuterocanonical Book of Judith.
Holofernes may also refer to: Holofernes (character), a schoolmaster in Shakespeare's Love's Labour's Lost Judith Holofernes (born 1976), German musician and lyricist
See also Judith and Holofernes (disambiguation) Pericopis holofernes, a synonym of the moth Dysschema terminata