There are at least 16 current and 19 former special routes of U.S. Route 27 (US 27), along with 10 current and six former special routes of State Route 1 (SR 1) in Georgia.

Florida

Sebring–Avon Park alternate route

U.S. Highway 27 Alternate (US 27 Alt.) existed from Sebring to Avon Park, Florida. It was deleted in 1979 and is now part of State Road 17 (SR 17).

Sebring–Leesburg temporary route

Haines City–Sunray Deli Estates alternate route

U.S. Highway 27 Alternate (US 27 Alt.) existed in Polk County from Haines City to Sunray Deli Estates, Florida. This bannered route was deleted in 1998, although some road maps and atlases still continued to show it until 2011. The road is now known as SR 17.

Lady Lake–Belleview alternate route

U.S. Highway 27 Alternate (US 27 Alt.) was an alternate route of US 27 that existed around Lake Weir, from Belleview to Lady Lake, existing until 1980. This was also concurrent with US 441 Alt. and today is a bi-county maintained segment of County Road 25 (CR 25). US 27 Alt. was the main route of US 441/US 27 prior to 1960.

Williston–Perry alternate route

U.S. Highway 27 Alternate (US 27 Alt.) is a alternate route of US 27 that exists in the north-central part of the U.S. state of Florida. It is signed north–south but is a primarily east–west route, traveling between Williston and Perry. US 27 Alt. is concurrent with US 19/US 98 from Chiefland to Perry and also has the hidden Florida Department of Transportation designation of SR 55, accounting for nearly two thirds of its route. Between Williston and Chiefland, it has the hidden SR 500 designation.

US 27 Alt. is one of the primary routes between Northwest and Central Florida, having been recently upgraded to four lanes along its entire length. With a signed speed limit of , this gives it more capacity than the mostly two-lane mainline route.

Major intersections

Georgia

Attapulgus business loop

U.S. Highway 27 Business (US 27 Bus.) is a business route of US 27 that partially exists in the city limits of Attapulgus, Georgia. It is concurrent with State Route 1 Business (SR 1 Bus.) for its entire length. US 27 Bus./SR 1 Bus. begin at an intersection with US 27/SR 1 east-southeast of Attapulgus. It travels to the west-southwest and curves to the northwest. At the city limits of Attapulgus, it curves to a due-west direction. It travels just south of Lillian E. Williams Elementary School and then intersects the northern terminus of SR 241 (South Main Street). It curves to a nearly due-north direction before leaving the city. It travels through rural areas of the county before a curve to the northeast. It meets its northern terminus, a second intersection with US 27/SR 1 (Tallahassee Highway), which is also the southern terminus of Hodges–Riley Road. The entire length of US 27 Bus./SR 1 Bus. is part of the National Highway System, a system of routes determined to be the most important for the nation's economy, mobility, and defense.

The roadway that would eventually become US 27 Bus./SR 1 Bus. was established at least as early as 1919 as part of SR 1. In the first half of 1936, this segment had a "completed hard surface". Between the beginning of 1953 and the 1960, SR 1 was routed on a more direct path between Amsterdam and Attapulgus, with a "soil surface". In 1989, an eastern bypass of Attapulgus, designated as SR 831, was proposed from east-southeast of the city to north-northwest of it. In 1995, US 27/SR 1 was shifted onto this bypass. Its former path was redesignated as SR 1 Bus. Between the beginning of 1996 and the beginning of 2011, US 27 Bus. was designated on the path of SR 1 Bus.

Major intersections

Bainbridge business loop

U.S. Highway 27 Business (US 27 Bus.) is a business route of US 27 that exists completely within the city limits of Bainbridge, Georgia. It is concurrent with State Route 1 Business (SR 1 Bus.) for its entire length. It begins at an interchange with US 27/US 84/SR 1/SR 38, which is a freeway-grade bypass of downtown Bainbridge. US 27 Bus./SR 1 Bus. travels to the north-northwest on Tallahassee Highway. The name changes to Scott Street at an intersection with College Street. At Shotwell Street, they begin a concurrency with US 84 Bus./SR 38 Bus. The four highways head on a due-west direction. At South Broad Street, US 27 Bus./SR 1 Bus. ends its concurrency with US 84 Bus./SR 38 Bus. and begins one with SR 97/SR 309. They pass Willis Park and then split at Calhoun Street, with SR 97/SR 309 heading east and US 27 Bus./SR 1 Bus. heading west. They curve to the west-northwest and cross over the Flint River. After this crossing, the local name changes to Dothan Road. At Newton Road, they begin a brief concurrency with SR 253; at Spring Creek Road, SR 253 splits off. They then reach their northern terminus, another interchange with the US 27/US 84/SR 1/SR 38 bypass. Here, US 84/SR 38 takes on the Dothan Road name; US 27/SR 1 uses the Colquitt Highway name. The roadway that would eventually become US 27 Bus./SR 1 Bus. was established at least as early as 1919 as part of SR 1. By the end of 1934, US 27 was designated on SR 1 in the area. By the end of 1939, this segment of US 27/SR 1 had a "completed hard surface". Between the beginning of 1960 and the beginning of 1965, US 27 (and possibly SR 1) in the Bainbridge area was shifted onto the bypass, at the time designated as SR 38 Loop. The former path of US 27 was redesignated US 27 Bus. By the beginning of 1977, SR 1 Bus. was indicated to be designated on US 27 Bus. in the city. In 1976, SR 38 Loop was decommissioned, with the SR 38 mainline designated on its former path. The former path of SR 38 was redesignated as SR 38 Bus.

Major intersections

Blakely business loop

U.S. Highway 27 Business (US 27 Bus.) is a business route of US 27 that exists completely within the city limits of Blakely, Georgia. It travels concurrent with State Route 1 Business (SR 1 Bus.) for its entire length. US 27 Bus./SR 1 Bus. begins at an intersection with US 27/SR 1 in the southeastern part of the city. They travel northwest on South Main Street and curve to a nearly due-north direction just before an intersection with SR 39 (East South Boulevard). US 27 Bus./SR 1 Bus./SR 39 continue to the north. Just to the east of Early County High and Middle schools, SR 62 (Columbia Street) joins the concurrency. This intersection also marks the western terminus of Bob White Avenue. Two blocks later, the concurrency begins to travel around the town square. On the east side of this square, SR 62 departs the concurrency. The concurrent highways continue northward, crossing over some railroad tracks of Norfolk Southern Railway and intersect SR 62 Byp. (Martin Luther King Jr. Boulevard). At this intersection, SR 39 departs the concurrency. US 27 Bus./SR 1 Bus. begins curving to the northwest and head to the east-southeast just before reaching their northern terminus, a second intersection with US 27/SR 1. Between the beginning of 1920 and the end of 1921, the Colquitt–Cuthbert section, which formerly traveled through Arlington and Edison, was shifted westward to travel through Blakely. By the end of 1934, US 27 was designated on all of SR 1. By the end of 1939, all of US 27/SR 1 in the Blakely area had a "completed hard surface". In 1992, an eastern bypass of Blakely, designated as SR 838, was proposed from south-southeast of the city to north-northeast of it. The next year, US 27/SR 1 in the Blakely area was shifted eastward, onto the path of SR 838. The former path through the city became US 27 Bus./SR 1 Bus.

Major intersections

Cuthbert business loop

U.S. Highway 27 Business (US 27 Bus.) is a business route of US 27 that partially exists in the city limits of Cuthbert, Georgia. It is concurrent with State Route 1 Business (SR 1 Bus.) for its entire length. US 27 Bus./SR 1 Bus. starts at an intersection with US 27/SR 1 south of Cuthbert. They travel on Blakely Street to the northwest and curve to the north-northeast. Just south of the city limits, they intersect the northern terminus of Edison Highway, which leads to SR 216. They cross over some railroad tracks of Norfolk Southern Railway. In downtown, they intersect US 82/SR 50 at the town square (which functions like a traffic circle around a memorial park). The four highways have a brief concurrency around the town square. After departing the town square, US 27/SR 1 curves to the north-northwest before resuming its north-northeast direction. After crossing over Town Branch, US 27 Bus./SR 1 Bus. leaves the city limits. It curves to the northeast and reaches its northern terminus, a second intersection with US 27/SR 1. The roadway that would eventually become US 27 Bus./SR 1 Bus. was established at least as early as 1919 as part of SR 1 from Edison, through Cuthbert, and into Lumpkin. By the end of 1921, the Colquitt–Cuthbert segment was shifted westward to travel through Blakely. By the end of 1934, US 27 was designated on the entire length of SR 1. In 1938, the northern two-thirds of the Randolph County portion of the Blakely–Cuthbert segment had a "completed hard surface". In the third quarter of 1939, the southern part of the Randolph County portion of the Cuthbert–Lumpkin segment also had a completed hard surface. In 1993, a southeastern bypass of Cuthbert, designated as SR 847, was proposed from south-southwest of the city to north-northeast of it. The next year, the path of US 27/SR 1 through the Cuthbert area was shifted eastward, onto SR 847. The former path was redesignated as US 27 Bus./SR 1 Bus.

Major intersections

Lumpkin connector route

State Route 1 Connector (SR 1 Conn.) is a connecting route of SR 1 that exists almost entirely within the city limits of Lumpkin, Georgia. It begins at an intersection with SR 27 (Broad Street) in the west-central part of the city. It travels nearly due north on Chestnut Street for one block to an intersection with the eastern terminus of SR 39 Conn. (Florence Street). It curves to the north-northeast and crosses over some railroad tracks of CSX Transportation before leaving the city. Almost immediately, it curves to the northwest and reaches its northern terminus, an intersection with US 27/SR 1. In 2001, SR 1 Conn. was established on its current path.

Major intersections

Columbus business loop

State Route 1 Business (SR 1 Bus.) was a business route of SR 1 that existed mostly within Columbus, Georgia. The roadway that would eventually become SR 1 Bus. was established at least as early as 1919 as part of SR 1 in the Columbus metropolitan area. Between the end of 1926 and the end of 1929, the entire length of highway in the area had a "completed hard surface". Between the beginning of 1953 and the beginning of 1964, a freeway in the eastern part of Columbus was under construction from US 27/US 280/SR 1 (Victory Drive) southeast of the city to SR 357 (Buena Vista Road). It was projected from there to US 27/SR 1 north-northeast of the city. In 1966, SR 1 was designated on this freeway. Its former path through Columbus was redesignated as SR 1 Bus. In 1975, this freeway was redesignated as part of Interstate 185 (I-185; with the unsigned designation SR 411). SR 1 was reverted onto its former path, replacing SR 1 Bus.

Major intersections

Columbus spur route 1