Uncharacterized protein C1orf21, also known as Proliferation-Inducing Protein 13, is a protein that in humans is encoded by the C1orf21 gene. C1orf21 is an intracellular protein that flows between the nucleus and the cytoplasm in the cell. It has been linked with cell growth and reproduction and there has been strong links with various types of cancers. There are no paralogs for this gene, however, many conserved orthologs have been found in all invertebrates. C1orf21 has low to moderate level of expression in most tissues in humans, however, it has the most expression in the skin, lung and prostate.
Gene Locus C1orf198 is a protein-encoding gene found on the reverse strand of chromosome 1 at the locus 1q25.3.
Gene neighborhood C1orf21 is located on the long arm of chromosome 1. It is found at position 5q23.1.
Cytogenic band: 1q25.3
Size Chromosome one is one of the longest chromosomes, in which C1orf21 spans from 184,385,826 to 184,390,390 bases, resulting with mRNA transcript that is 10,278 nucleotides long with 4 exons. The protein is 121 amino acids long, containing a domain of unknown function known as DUF4612.
Expression NCBI gene and RNA-Seq revealed that C1orf21 is expressed in all tissues at a low to moderate level, however, it is mostly expressed in the skin, brain and prostate.
Gene level regulation Promoter There was over 7 promoters that were predicted, but the true promoter was 1111 base pairs long known as .
Transcription factor binding sites Many transcription factor (TF) binding sites have been predicted through Genomatix. Some important binding cites include MYRE, MARs, and Bright.
MYRE is a myelin regulatory factor. Myelin is produced in the central nervous system and plays a large role in axons. MARs is a special AT-rich sequence-binding protein 1, predominantly expressed in thymocytes, binds to matrix attachment regions. Bright helps with B cell regulator of IgH transcription.
Protein Subcellular location It was predicted that the location of C1orf21 is in the nucleus with 62.2% certainty. The mitochondria was predicted at 17.4%: mitochondrial, while the cytoskeleton, and vascular system at 4.3%.
Structure C1orf21 protein is 121 amino acids long with a molecular weight of 18,7 kDa with an isoelectric point of 5.08. It is believed that the protein interacts with the nuclear membrane and contains an unknown domain known as DUF4612. For the secondary and tertiary structure it is predicted that there are many alpha helices in the structure, with the rest of the protein having a disordered structure.
Protein level regulation O-glycosylation sites: Serine 5, Threonine 11, Serine 66, Serine 68, and Serine 69. Palmitioyaltion site: Cysteine 3 Phosphorylation: Serine 34, Serine 44, Serine 66, Serine 69, Serine 75, Serine 95, Serine 115, Serine 121 Sumoylation site: Lysine 46 and Lysine 106 Tyrosine sulfation site: Tyrosine 113
Interacting proteins Protein Function Calcineurin-binding protein cabin-1 (Cabin1)Required for replication-independent chromatin assemblyCentrosomal protein of 162 kDa (CEP162)Required to promote assembly of the transition zone in primary cilia.CD97 antigenReceptor potentially involved in both adhesion and signaling processes early after leukocyte activation.Chromosome 11 open reading frame 57 (C11orf57)UnknownChromosome 5 open reading frame 51 (C5orf51)UnknownHomeobox protein Nkx-2.8; (NKX2-8)NKL subclass homeoboxes and pseudogenesNACHT, LRR and PYD domains-containing protein 13 (NLPR13)Involved in inflammationSemaphorin-3C (SEMA3C)Binds to plexin family members and plays an important role in the regulation of developmental processesZinc finger protein 19 (ZNF19)transcriptional regulation
Homology Paralogs
There are no isoforms or paralogs of C1orf21 that are known.
Orthologs C1orf21 is found in most classes of vertebrates and some invertebrates. The most distant ortholog of C1orf21 is Acropora digitifera, which diverged an estimated 824 million years ago. There is no traces of the C1orf21 gene in organisms that are traced beyond invertebrates, such as fungi, plants, protists, or single celled organisms.
Homologous domains The domain of unknown function 4612 (DUF4612) was highly conserved in most orthologs. SpeciesCommon nameTaxonomic groupDOD (MYA)Accession numberSequence length (aa)IdentitySimilarityHomo sapiensHumanPrimates0NP_110433121100100Pan troglodytesChimpanzeePrimates7NP_001229539121100100Gorilla gorilla gorillaGorillaPrimates9XP_018883443121100100Macaca mulattaRhesus macaquePrimates30NP_001247792121100100Cercocebus atysSooty mangabeyPrimates30XP_011903171121100100Ursus maritimusPolar bearCarnivora96XP_0086953661219799Pogona vitticepsCentral bearded dragonAmphioxiformes312XP_0206507641219497Gallus gallusRed junglefowlGalliformes312XP_4222921219398Haliaeetus leucocephalusBald eagleAccipitriformes312XP_0105789921219398Fulmarus glacialisNorthern fulmarProcellariiformes312KFV96345909398Ophiophagus hannahKing cobraSquamata312ETE667281219196Xenopus tropicalisWestern clawed frogAnura352NP_0010726521217785Nothobranchius furzeriTurquoise killifishCyprinodontiformes435XP_0158270001166173Echeneis naucratesLive sharksuckerPerciformes435XP_0293557621166173Haplochromis burtoniBurton's mouthbrooderCichliformes435XP_0059325281166173Anabas testudineusBlue perchAnabantiformes435XP_0262017021164760Callorhinchus miliiAustralian ghostsharkChimaeriformes473XP_0078937871356979Rhincodon typusWhale SharkOrectolobiformes473XP_020373635916882Branchiostoma belcheriBelcher's lanceletAmphioxiformes684XP_0196409801143356Acropora digitiferaStony coral pulpScleractinia824XP_0157472271405565
Function C1orf21 is most likely involved in the growth of cells, especially in the nucleus where replication of DNA occurs.
Clinical significance Even though there is not a lot known about C1orf21, there have been some links with diseases. In many studies it has been found that there are links with cancer. Since C1orf21 is associated with cell proliferation, in another study by Sooda et al. there was an interest in the transcript map of the HPC1 locus, to help them identify the susceptibility genes involved in prostate cancer and jaw tumor. It was seen that overall there are several studies where C1orf21 has been studied on role it plays in cancer for different body areas among many other genes. It was also found that there is a large correlation with affects on keratinocytes since C1orf21 plays a role in ZNF750 silencing.
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