Melbourne's bus network is a major transport system in Australia, comprising around 400 bus routes. The bus network is run by private companies under contract to Public Transport Victoria. Buses in Melbourne use the Myki ticketing system, with the exception of SkyBus services.

Kinetic Melbourne operate approximately 30% of the bus network, signing contracts in 2021 as part of the Melbourne Metropolitan Bus Franchise. The remaining 70% of the network is operated under 23 contracts with other bus operators, all of these contracts were entered into in mid-2018 and will expire in 2025 or 2028.

All bus routes are allocated a number from 140-999, with the exception of SkyBus services. Route numbers below 140 are used by trams. The numbering of a route can indicate the geographical location served, the route's history, or the route's type of operation; for example, routes from the 900 series were historically used by the Victorian Railways, whose buses coordinated with trains on some routes, but now the series is used for the SmartBus and Night Network routes.

SkyBus services run to and from Melbourne and Avalon airports.

Some bus routes have been taken out of operation. They may have been scrapped, replaced (either by another route or a FlexiRide service), incorporated into existing routes, or split. Reasons may include low patronage, confusing routing, or infrequent service patterns.

List of operational routes by number 100–199 + RouteImageDestinationsOperator(s)Notes109Queen Bridge Street tram stop Station PierVentura Bus LinesSupplementary service to tram route 109 when cruise ships are docked at Station Pier140ARockbank stationvia Altezze DriveMount AtkinsonTransit Systems VictoriaCommenced 28 June 2026. To be extended to Tarneit station as route 140.150Williams Landing stationvia Sayers Road Tarneit stationCDC MelbourneCommenced 21 July 2015 to replace part of former route 446, operates all night on Friday & Saturday nights as part of the Night Network151Williams Landing stationvia Westmeadows Lane Tarneit stationCDC MelbourneCommenced 21 July 2015, replaced part of former routes 444 and 446152Williams Landing stationvia Palmers Road Tarneit stationCDC MelbourneCommenced 30 May 2021153Williams Landing stationvia Princes Highway Werribee stationCDC MelbourneCommenced 21 July 2015, replaced former routes 433, 443, and 493154 Tarneit stationvia Truganina Laverton stationCDC MelbourneCommenced 7 December 2025160Hoppers Crossing stationvia Morris Road Tarneit stationCDC MelbourneCommenced 21 July 2015, replaced former route 444161Hoppers Crossing stationvia Pacific Werribee Werribee stationCDC MelbourneCommenced 21 July 2015, replaced former routes 436 and 437166Hoppers Crossing stationvia Pacific Werribee Wyndham Vale stationCDC MelbourneCommenced 21 July 2015, replaced routes 447 and 448167Hoppers Crossing stationvia Pacific Werribee Tarneit stationCDC MelbourneCommenced 21 July 2015, replaced route 442170Werribee stationvia Pacific Werribee Tarneit stationCDC MelbourneCommenced 21 July 2015180Werribee stationvia Tarneit Road Tarneit stationCDC MelbourneCommenced 21 July 2015, operates all night on Friday & Saturday nights as part of the Night Network181Werribee stationvia Pacific Werribee Hoppers Crossing stationCDC MelbourneCommenced 21 July 2015, replaced former routes 445 and 446182Werribee stationvia Tarneit West Tarneit StationCDC MelbourneCommenced 30 May 2021, rerouted via West Tarneit station on 13 September 2026186West Tarneit stationvia Dohertys Road Tarneit StationCDC MelbourneCommences 13 September 2026190Werribee stationvia Ballan Road Wyndham Vale stationCDC MelbourneCommenced 21 July 2015, operates all night on Friday & Saturday nights as part of the Night Network191Werribee stationvia Greaves Street Jubilee estate, Wyndham ValeCDC MelbourneCommenced 21 July 2015, replaced former route 448192Werribee stationvia Black Forest Road Wyndham Vale stationCDC MelbourneCommenced 21 July 2015, replaced former route 449194Harpley estate (Werribee)via Mambourin Wyndham Vale stationCDC MelbourneCommenced 7 December 2025, extending to Werribee station from 13 September 2026

200–299 + RouteImageDestinationsOperator(s)Notes200City (Queen Street)via Kew JunctionBulleenKinetic Melbourne201Box Hill stationDeakin UniversityVentura Bus LinesShuttle service to Deakin University Burwood207City (Queen Street)via Kew JunctionWestfield DoncasterKinetic MelbourneOperates all night on Friday & Saturday nights as part of the Night Network215Caroline SpringsHighpoint Shopping CentreKinetic Melbourne216Sunshine stationvia Footscray, Dynon RoadCity (Queen Street)Kinetic MelbourneFormerly ran between Sunshine & Brighton Beach until 16 November 2019 (now route 603).220Sunshine stationvia Footscray, Footscray Road, Queen Victoria MarketCity (Queen Street)Kinetic MelbourneFormerly ran between Sunshine & Gardenvale until 16 November 2019 (now route 604).223Yarravillevia FootscrayHighpoint Shopping CentreKinetic Melbourne232Altona NorthQueen Victoria MarketKinetic Melbourne234Garden CityQueen Victoria MarketKinetic Melbourne235Southern Cross stationvia Williamstown RoadFishermans BendKinetic Melbourne236Garden Cityvia South MelbourneQueen Victoria MarketKinetic Melbourne237Southern Cross stationvia Lorimer StFishermans BendKinetic Melbourne241North Melbourne stationvia Melbourne UniversityYarra Bend ParkTransit Systems VictoriaCommenced 1 February 2026, replaced former route 202 and 401246Elsternwick stationvia St KildaClifton HillKinetic Melbourne250City (Queen Street)via Clifton HillLa Trobe University, BundooraKinetic MelbourneOperates all night on Friday & Saturday nights as part of the Night Network251City (Queen Street)via Clifton HillNorthland Shopping CentreKinetic Melbourne270Box Hill stationvia Blackburn NorthMitchamKinetic MelbourneBox Hill stationvia Park OrchardsRingwoodKinetic Melbourne273The Pines Shopping CentreNunawading stationKinetic Melbourne279Box Hill stationvia Middleborough RoadWestfield DoncasterorTemplestoweKinetic Melbourne280The Pines Shopping CentreWestfield DoncasterThe Pines Shopping CentreKinetic MelbourneClockwise circular service281Templestowevia Westfield DoncasterBox Hill stationKinetic Melbourne Route truncated from Deakin University to Box Hill station from 16 October, 2023282The Pines Shopping CentreWestfield DoncasterThe Pines Shopping CentreKinetic MelbourneAnti-clockwise circular service284Box Hill stationvia Union RoadDoncaster Park & RideKinetic Melbourne Route truncated from Westfield Doncaster to Doncaster Park & Ride285Doncaster Park & Ridevia North BalwynCamberwellKinetic Melbourne Route truncated from Westfield Doncaster to Doncaster Park & Ride293Box Hill stationvia Westfield DoncasterGreensborough stationKinetic Melbourne295Westfield DoncasterThe Pines Shopping CentreKinetic Melbourne Route truncated from Box Hill station to Westfield Doncaster

300–399 + RouteImageDestinationsOperator(s)Notes301Reservoir stationLa Trobe University (Bundoora Campus)DysonsUniversity Shuttle BusCity (Lonsdale Street)via Belmore Road, Eastern FreewayBox Hill stationKinetic MelbourneOriginally had an extension to Crown Melbourne303City (Queen Street)via Park RdRingwood NorthKinetic MelbourneCeased 27 July 2014 Reinstated 13 October 2014304City (Lonsdale Street)via Belmore Road, Eastern FreewayWestfield DoncasterKinetic MelbourneCity (Lonsdale Street) (peak)via Eastern FreewayorWestfield Doncaster (off-peak)via George StreetThe Pines Shopping CentreKinetic MelbourneOriginally ran to Warrandyte BridgeCity (Queen Street)via Eastern Freeway, Reynolds RoadThe Pines Shopping CentreKinetic MelbourneCity (Lonsdale Street)Deep CreekKinetic Melbourne343Hurstbridge stationvia Diamond Creek stationGreensborough stationDysonsCity (Queen Street)via Eastern FreewayLa Trobe University, BundooraKinetic Melbourne356Epping stationWollert EastDysons357Wollert WestThomastown stationDysonsOperates all night on Friday & Saturday nights as part of the Night Network358EppingWollertDysonsWarrandyteRingwoodKinetic MelbourneMitchamvia Ringwood NorthRingwoodKinetic Melbourne381Diamond Creek stationMernda stationDysons382Whittleseavia South Morang stationNorthland Shopping CentreDysons383PalisadesUniversity HillDysons384KinglakeWhittleseaDysonsThe majority of services on this route run to the eastern end of Whittlesea, with 5 weekday services going to Kinglake and 2 making the trip to the next town along the route at Humevale.385Whittlesea / Mernda stationGreensborough stationDysons386Mernda stationvia Cravens RoadBundoora RMITDysonsOperates all night on Friday & Saturday nights as part of the Night Network387Mernda stationvia Hawkstowe ParadeBundoora RMITDysons388Mernda LoopDysonsAnti-clockwise circular service389Mernda LoopDysonsClockwise circular service390Merndavia WollertCraigieburnDysonsCommenced 31 October 2021

400–499