Prüfer (German for "examiner") is a German surname. Non-German language versions are Pruefer or Prufer. Notable people with these surnames include:

Arthur Prüfer (1860–1944), German musicologist Clemens Prüfer (born 1997), German discus thrower, brother of Henning Cornelia Prüfer-Storcks (born 1956), German politician Curt Prüfer (1881–1959) German diplomat Gustav Franz Pruefer (1861–1951), American music instrument inventor Heinz Prüfer (1896–1934), German Jewish mathematician Henning Prüfer (born 1996), German discus thrower Kevin Prufer (born 1969), American poet, novelist, academic, editor, and essayist Thomas Prufer (1929–1993), American philosopher

See also Jan Prüffer (1890–1959), Polish biologist