Nafpliotis () is a Greek surname which means "descending from Nafplio" (toponymic surname). The female equivalent is Nafpliotou (. Notable people with the surname include:
Iakovos Nafpliotis (1864–1942), the Archon Protopsaltis of the Holy and Great Church of Christ in Constantinople Ioannis Nafpliotis (born 1970), Greek sprinter Maria Nafpliotou (born 1969), Greek actress