The following highways are numbered 381:
Brazil BR-381
Canada Newfoundland and Labrador Route 381 Quebec Route 381 Saskatchewan Highway 381
Cuba
Road of Jamaica (Cuba) (2–381)
Japan Japan National Route 381
United States Interstate 381 Georgia State Route 381 (former) Kentucky Route 381 Maryland Route 381 New York State Route 381 (former) North Carolina Highway 381 Pennsylvania Route 381 Puerto Rico Highway 381 South Carolina Highway 381 Tennessee State Route 381 Virginia State Route 381