Ruffner is a surname. Notable people with the surname include:
Clark L. Ruffner (1903–1982), U.S. Army general Frederick Gale Ruffner Jr. (1926–2014), American publisher Ginny Ruffner (1952–2025), American glass artist Henry Ruffner (1790–1861), American educator and Presbyterian minister Lewis Ruffner (1797–1883), American merchant, magistrate, slaveowner, and politician Mason Ruffner (born 1947), American blues and rock singer, guitarist, and songwriter Pattie Ruffner Jacobs (1875–1935), American suffragist Paul Ruffner (1948–2022), American basketball player Viola Ruffner (1812–1903), American schoolteacher