John Best may refer to:
Politicians John Best (died 1560), MP for Westminster John Best (died 1574), in 1553 MP for Colchester John Best (British politician) (1821–1865), British barrister and politician John Best (Canadian politician) (1861–1923), Australian-born politician in Canada
Sports John Best (basketball) (born 1971), American basketball player Johnny Best (boxer), 1940s Australian middleweight boxer John Best (soccer) (1940–2014), US/English soccer defender John Orr Best (1910–1996), American soccer referee and member of the National Soccer Hall of Fame Jack Best (rugby union) (1914–1994), New Zealand rugby union player John Best (badminton) (born 1929), English badminton player
Others John Best (bishop) (died 1570), Bishop of Carlisle 1560–1570 John Best (guard captain), 1592–1597 Captain of the Yeomen of the Guard John C. Best (1865–1902), Canadian murderer John William Best (1912–2000), Royal Air Force pilot Johnny Best (1913–2003), American jazz trumpeter