Ruffini is an Italian surname. Notable people with the surname include:
Angelo Ruffini (1864–1929), Italian histologist and embryologist Attilio Ruffini (1924–2011), Italian politician Ernesto Ruffini (1888–1967), Archbishop of Palermo Frederick Ernst Ruffini (1851-1885), American architect Giovanni Ruffini (1807–1881), Italian poet and librettist Giulano Ruffini (born 1945), French art collector Giuseppe or Joseph Ruffini (1690–1749), Italian-Austrian painter Luca Ruffini (born 1997), Italian footballer Oscar Ruffini (1858-1957), American architect Paolo Ruffini (mathematician) (1765–1822), Italian mathematician and philosopher Paolo Ruffini (actor) (born 1978), Italian actor and presenter Patrick Ruffini, political pollster and strategist Sandro Ruffini (1889–1954), Italian actor and voice actor Remo Ruffini (born 1942), Italian astrophysicist Remo Ruffini (businessman) (born 1961), Italian billionaire businessman Silvia Ruffini (1475–1561), Italian noble woman and mistress of Cardinal Alessandro Farnese Simone Ruffini (born 1989), Italian swimmer Stefano Ruffini (1963–2006), Italian singer and voice actor
See also Eugen Ruffínyi (1846–1924), Slovak mining engineer and amateur speleologist; the original family name was probably "Ruffini"