Phosphoribokinase () is an enzyme that catalyzes the chemical reaction

The enzyme characterised from rabbit converts the pentose sugar, D-ribose 5-phosphate (shown in its open-chain aldehydo form), to α-D-ribose 1,5-bisphosphate by transferring a second phosphate group from the cofactor, adenosine triphosphate (ATP), which is converted to adenosine diphosphate (ADP).

This enzyme is a transferase, specifically one transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:D-ribose-5-phosphate 1-phosphotransferase. This enzyme is also called phosphoribokinase (phosphorylating).

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