Diphosphate-purine nucleoside kinase () is an enzyme that catalyzes the general chemical reaction
diphosphate + a purine nucleoside phosphate + a purine mononucleotide
For example, it can convert guanosine to guanosine monophosphate:
The enzyme was characterised from the Acholeplasma class of Mollicutes.
This enzyme is a transferase, specifically one transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is diphosphate:purine nucleoside phosphotransferase. This enzyme is also called pyrophosphate-purine nucleoside kinase.
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