Trehalose 6-phosphate phosphorylase () is an enzyme that catalyzes the chemical reaction

The two substrates of this enzyme characterised from Lactococcus lactis are alpha,alpha'-trehalose 6-phosphate and orthophosphate (Pi). Its products are glucose 6-phosphate and glucose 1-phosphate.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is alpha,alpha-trehalose 6-phosphate:phosphate beta-D-glucosyltransferase. This enzyme is also called trehalose 6-phosphate:phosphate beta-D-glucosyltransferase.

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