MacIsaac is a surname. Notable people with the surname include:
Angus MacIsaac (born 1943), English educator and businessman Angus MacIsaac (footballer) (1900-1944) Australian rules footballer Ashley MacIsaac (born 1975), Canadian fiddler Dave MacIsaac (born 1955), Canadian musician Dugald MacIsaac (1901-1961), Scottish chess player , Canadian zoologist Martha MacIsaac (born 1984), Canadian actress Veronica MacIsaac, Canadian fashion designer who specialises in tartan