alpha,alpha-trehalose phosphorylase () is an enzyme that catalyzes the chemical reaction

The two substrates of this enzyme characterised from Euglena gracilis are trehalose and orthophosphate (Pi). Its products are β-D-glucose-1-phosphate and D-glucose. The enzyme can act in reverse, producing the disaccharide trehalose.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is alpha,alpha-trehalose:phosphate beta-D-glucosyltransferase. This enzyme is also called trehalose phosphorylase.

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