Glucosamine N-acetyltransferase () is an enzyme that catalyzes the chemical reaction

The two substrates of this enzyme are D-glucosamine and acetyl-CoA. Its products are N-acetylglucosamine and coenzyme A.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:D-glucosamine N-acetyltransferase. Other names in common use include glucosamine acetylase, and glucosamine acetyltransferase. It participates in the metabolism of amino sugars.

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