Pentanamidase () is an enzyme characterised from Alcaligenes eutrophus which catalyzes a hydrolysis reaction converting valeramide (pentanamide) to valeric acid and ammonia:
This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is pentanamide amidohydrolase. This enzyme is also called valeramidase.
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