N,N-dimethylformamidase () is an enzyme characterised from a Pseudomonas species that catalyzes the hydrolysis of dimethylformide to formic acid and ammonia:

It has been suggested that this enzyme could be a component of a biological system to remove dimethylformamide from industrial waste water.10.1021/acs.jafc.2c05240

This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N,N-dimethylformamide amidohydrolase. Other names in use include dimethylformamidase, and DMFase. It is a non-heme iron protein.

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