Hippurate hydrolase () is an enzyme that catalyzes the chemical reaction
The enzyme characterised from Bacillus species and Pseudomonas putida hydrolyses hippuric acid to benzoic acid and glycine. The enzyme contains zinc as Zn2+.
A test for hippurate hydrolysis has been used to identify pathogens such as Gardnerella vaginalis, Campylobacter jejuni, Listeria monocytogenes and group B streptococci which produce the enzyme.
This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-benzoylamino-acid amidohydrolase. It is also called N-Benzoylglycine amidohydrolase.
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