D-glutaminase () is an enzyme that catalyzes the chemical reaction

The enzyme characterised from Blastocladiella emeronii and Aspergillus oryzae hydrolyses D-glutamine to D-glutamic acid and ammonia.

This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is D-glutamine amidohydrolase.

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