Citrullinase () is an enzyme that catalyzes the chemical reaction
The enzyme characterised from Tetrahymena pyriformis hydrolyses citrulline to the amino acid, ornithine, with ammonia and carbon dioxide as byproducts.
This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is L-citrulline N5-carbamoyldihydrolase. Other names in use include citrulline ureidase, citrulline hydrolase, and L-citrulline 5-N-carbamoyldihydrolase.
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