Carboxymethylhydantoinase () is an enzyme characterised from the soil bacterium, Zymobacterium oroticum, that catalyzes the hydrolysis of L-5-carboxymethylhydantoin to N-carbamoyl-L-aspartic acid:
The reaction is specific to the L-5-carboxymethylhydantoin enantiomer and hence it can be used in a sequence to manufacture natural L-aspartic acid from the racemic hydantoin.
This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is L-5-carboxymethylhydantoin amidohydrolase. This enzyme is also called hydantoin hydrolase.
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