5-aminopentanamidase () is an enzyme that catalyzes the chemical reaction

The enzyme characterised from Pseudomonas putida hydrolyses 5-aminopentanamide to 5-aminopentanoic acid and ammonia. The reaction is part of the breakdown pathway for the amino acid, lysine.

This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is 5-aminopentanamide amidohydrolase. Other names in common use include 5-aminovaleramidase, and 5-aminonorvaleramidase.

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