The enzyme steryl-β-glucosidase () catalyzes the following chemical reaction:

The enzyme characterised from Sinapis alba hydrolyses cholesteryl β-D-glucoside to cholesterol and D-glucose.

It is a hydrolase, specifically a glycosidase that hydrolyses O- and S-glycosyl compounds. The systematic name is cholesteryl-β-D-glucoside glucohydrolase.

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