The enzyme GDP-glucosidase () catalyzes the chemical reaction

The enzyme characterised from yeast hydrolyses guanosine diphosphate glucose (GDP-glucose) to D-glucose and guanosine diphosphate.

This enzyme is a hydrolase, specifically a glycosidase that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is GDP-glucose glucohydrolase. Other names in use include guanosine diphosphoglucosidase, and guanosine diphosphate D-glucose glucohydrolase.

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