The enzyme xylono-1,4-lactonase (EC 3.1.1.68) catalyzes the reaction

The enzyme characterised from Pseudomonas fragi and Gluconobacter oxydans hydrolyses D-xylono-1,4-lactone to D-xylonic acid. The enzyme is of interest as a target for metabolic engineering.

This enzyme is a hydrolase, specifically one acting on carboxylic ester bonds. The systematic name of this enzyme class is D-xylono-1,4-lactone lactonohydrolase. Other names in common use include xylono-γ-lactonase, and xylonolactonase.

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