Steroid-lactonase (EC 3.1.1.37) is an enzyme that catalyzes the reaction

The enzyme characterised from Cephalosporium acremonium hydrolyses the steroid, testololactone, to testolic acid.

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name testololactone lactonohydrolase.

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