Methylumbelliferyl-acetate deacetylase (EC 3.1.1.56, esterase D) is an enzyme that catalyzes the reaction

The enzyme characterised from humans hydrolyses 4-methylumbelliferyl acetate to hymecromone and acetic acid.

This enzyme is hydrolase, specifically one acting on carboxylic ester bonds. The systematic name is 4-methylumbelliferyl-acetate acylhydrolase. This enzyme is a member of the esterase D family.

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