4-pyridoxolactonase (EC 3.1.1.27) is an enzyme that catalyzes the reaction
The enzyme characterised from Pseudomonas species and Mesorhizobium loti hydrolyses 4-pyridoxolactone to 4-pyridoxic acid. Its structure has been determined by X-ray crystallography. The reaction is part of the breakdown of vitamin B6 and pyridoxine.
This enzyme is a hydrolase, specifically one acting on carboxylic ester bonds. The systematic name is 4-pyridoxolactone lactonohydrolase.
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