A flavonol 3-sulfotransferase () is an enzyme that catalyzes the general chemical reaction

It uses the cofactor 3'-phosphoadenosine-5'-phosphosulfate (PAPS) and gives adenosine 3',5'-bisphosphate as a byproduct. For example, the enzyme characterised from Flaveria chlorifolia converts quercetin to quercetin 3-O-sulfate :

This enzyme is a transferase of sulfotransferase type, which transfer sulfate groups. The systematic name of this enzyme class is 3'-phosphoadenylyl-sulfate:flavonol 3-sulfotransferase.

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