In enzymology, glycine dehydrogenase () is an enzyme that catalyzes the chemical reaction

The three substrates of this enzyme are glycine, water, and oxidised nicotinamide adenine dinucleotide (NAD+). Its products are glyoxylic acid, reduced NADH, ammonia, and a proton.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is glycine:NAD+ oxidoreductase (deaminating).

This should not be confused with:

the glycine dehydrogenase (decarboxylating), which is another name for the glycine cleavage system P-protein ().

or the glycine dehydroganse (cyanide forming) ().

or the glycine dehydrogenase (cytochrome) ().

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