Estradiol 6beta-monooxygenase () is an enzyme that catalyzes the chemical reaction:

Estradiol 6β-monooxygenase is an oxidoreductase, that uses molecular oxygen to introduce a hydroxy group at a specific position in the female sex hormone, estradiol. The systematic name of this enzyme class is estradiol-17beta,hydrogen-donor:oxygen oxidoreductase (6beta-hydroxylating). This enzyme is also called estradiol 6beta-hydroxylase.

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