Cholesterol 25-hydroxylase ( that in humans is encoded by the CH25H gene) is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are cholesterol, a reduced electron acceptor, and oxygen. Its products are 25-hydroxycholesterol, the electron acceptor, and water.
This enzyme is an oxidoreductase, that uses molecular oxygen as oxidant and contains a di-iron (Fe-O-Fe) cofactor. The systematic name of this enzyme class is cholesterol, hydrogen-donor:oxygen oxidoreductase (25-hydroxylating). It is also called cholesterol 25-monooxygenase.
Transcripts for this enzyme have been identified in macrophages from the testis.
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