Benzoate 4-monooxygenase (, Formerly ) is an enzyme that catalyzes the chemical reaction
The four substrates of this enzyme are benzoic acid, reduced nicotinamide adenine dinucleotide phosphate (NADPH), oxygen, and a proton. Its products are 4-hydroxybenzoic acid, oxidised NADP+, and water.
The enzyme is a cytochrome P450 protein containing heme. It requires a partner, cytochrome P450 reductase, using NADPH for functional expression. The systematic name of this enzyme class is benzoate,NADPH:oxygen oxidoreductase (4-hydroxylating). Other names in common use include benzoic acid 4-hydroxylase, benzoate 4-hydroxylase, benzoic 4-hydroxylase, benzoate-p-hydroxylase, and p-hydroxybenzoate hydroxylase. It participates in benzoate degradation via hydroxylation.
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