4-nitrophenol 2-monooxygenase () is an enzyme that catalyzes the chemical reaction
The four substrates of this enzyme are 4-nitrophenol, reduced nicotinamide adenine dinucleotide (NADH), oxygen. and a proton. Its products are 4-nitrocatechol, oxidised NAD+, and water.
The enzyme is a flavin-containing monooxygenase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. The systematic name of this enzyme class is 4-nitrophenol,NADH:oxygen oxidoreductase (2-hydroxylating). Other names in common use include 4-nitrophenol hydroxylase, and 4-nitrophenol-2-hydroxylase.
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