2-hydroxypyridine 5-monooxygenase () is an enzyme that catalyzes the chemical reaction

The three substrates of this enzyme are 2-hydroxypyridine (2-pyridone), a reduced electron acceptor, and oxygen. Its products are 2,5-dihydroxypyridine, the electron acceptor, and water.

This enzyme is an oxidoreductase, that uses molecular oxygen as oxidant. The systematic name of this enzyme class is 2-hydroxypyridine,hydrogen-donor:oxygen oxidoreductase (5-hydroxylating). This enzyme is also called 2-hydroxypyridine oxygenase.

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