Chambersburg, Pennsylvania is a borough in the South Central region of Pennsylvania.

Chambersburg may also refer to several places:

Chambersburg, Illinois Chambersburg, Indiana Chambersburg Township, Pike County, Illinois Chambersburg Township, Iredell County, North Carolina Chambersburg, Missouri Chambersburg, Trenton, New Jersey, a neighborhood within the South Trenton neighborhood in Trenton, New Jersey Chambersburg, Ohio Chambersburg Area Senior High School